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Fig. 2. Expression of full-length and CtBP-independent regions of the Knirps transcriptional repressor in transgenic Drosophila. (A) Structure of proteins expressed from hsp70 promoter: 1-429, full-length Knirps protein; 75-429, non-DNA binding control protein; 1-330, CtBP-independent Knirps repression domain; 75-330, non-DNA binding control protein. (B) In situ analysis of expression of knirps mRNA produced from hsp70-knirps transgene before and after heatshock. (C) (Top) Proteins expressed from representative lines of the four constructs measured by western blot. M2 {alpha}-FLAG antibody was used to detect recombinant proteins. Lines shown in lanes 2 and 4 were used in subsequent experiments. Lane 8, non heat shock control. (Bottom) Coomassie blue stained gel illustrates equal loading.





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