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Fig. 4. Differential repression of minimal eve stripe enhancers by
Knirps1-429 and 1-330, demonstrates differential sensitivities to Knirps
activity. Repression of eve stripe 2/3-lacZ reporter gene
demonstrates differential sensitivities of eve stripe 2 versus stripe
3 enhancers to Knirps expression. Patterns of lacZ expression in
embryos prior to heat shock (A,D) and after heat shock (B,C,E,F). After a
10-minute heat shock, the majority of embryos expressing Knirps 1-429 showed
repression of eve stripe 3 (B). After a 30-minute heat shock, the
majority of embryos showed repression of both stripe 2 and 3 (C). A
significant percentage of embryos showed only partial repression of stripe 3
upon overexpression of Knirps 1-330 (E). The majority of embryos demonstrated
a loss of stripe 3, but not stripe 2 after 30 minutes of heat shock (F). (G,J)
Unperturbed reporter gene expression. Effects of overexpression of Knirps1-429
and 1-330 in embryos carrying the eve stripe 3/7 lacZ
reporter (G-I) or eve stripe 4/6 lacZ reporter (J-L).
Full-length Knirps was a more potent repressor, but Knirps 1-330 was capable
of repressing the minimal eve stripe 4/6 element (see text for
details). The patterns shown in H and K are representative of embryos heat
shocked for 15 minutes, whereas the patterns shown in I and L are typical of
embryos heat shocked for 30 minutes. Embryos are oriented with anterior
towards the left, dorsal side upwards.
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