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Fig. 5. Quantitation of proteins expressed from hsp70-knirps transgenes
demonstrates that full-length Knirps 1-429 is less abundant than Knirps 1-330.
(A) Western blot analysis of embryos subjected to the same heat-shock regimen
(0, 5, 10 and 20 minutes) used for analysis shown in
Fig. 3 and
Table 1. Asterisk marks
nonspecific cross-reacting protein that was also present in lysates from
non-transgenic Drosophila (presence of nonspecific band appeared to
vary with batch of antibody; data not shown). Because this nonspecific band
co-migrates with the 1-330 protein, the signal from the nonspecific protein
(averaged from lanes 5-8) was subtracted from each of the values in lanes 1-4
to determine levels of 1-330 protein. Below, Coomassie stained gel showing
equal loading. (B) Quantitation of western blots demonstrates an approximately
twofold higher level of Knirps 1-330 protein at each time point than Knirps
1-429, demonstrating that the higher activity of the 1-429 is not due to
higher levels of this protein. Standard deviations are shown in B for four
separate gels and quantitations of the heat-shock experiment shown in A.
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