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Fig. 6. Effect of expression of Knirps proteins on hunchback
(hb), runt, hairy and fushi tarazu (ftz)
demonstrates differential activity of Knirps 1-429 versus Knirps 1-330 on all
but the most sensitive target genes. hb, a sensitive target of Knirps
(A-C), showed similar repression by both Knirps 1-330 and Knirps 1-429 of the
parasegment four zygotic expression pattern (arrow) after 30 minutes of heat
shock. On other genes, Knirps 1-330 was much less potent than Knirps 1-429.
Knirps 1-330 repressed only stripe 3 of runt (E), while Knirps 1-429
repressed all except runt stripe 5 (F) after 15 minutes of heat shock
(similar patterns were observed at 30 minutes). Similarly, 1-330 had a modest
effect only on hairy stripes 3/4 (H) and ftz stripe 3 (L),
while Knirps 1-429 completely repressed hairy 3,4 7 (I) and
extensively disrupted ftz expression (K) after 5 and 10 minutes of
heat shock respectively (similar patterns were noted at 15 and 30 minutes).
Transcripts of endogenous Knirps target genes were visualized by in situ
hybridization. All embryos are oriented with anterior towards the left, dorsal
upwards.
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