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Fig. 5. Nv-mef2. Phylogenetic analysis and gene expression. (A) Alignment
of 68 amino acids from the conserved MADS domain of various mef2
genes plus blistered and serum response factor (SRF). (B)
Phylogeny of mef2 sequences. The tree was constructed and labeled as
in Fig. 3. Phylogenetic
analysis was based on the amino acid sequence of the MADS domain. Sequences
included in the analysis are: blistered, Drosophila, residues
166-233; mef2 (myocyte enhancing factor 2), Drosophila,
residues 2-69; mef2, Nematostella, residues 2-69; mef2,
Podocoryne, residues 2-69; MEF2A (myocyte enhancer factor 2A),
Homo, residues 2-69; MEF2B, Homo, residues 2-69; MEF2C,
Homo, residues 2-69; MEF2D, Homo, residues 2-69; SRF
(serum response factor), Homo, residues 142-209. (C-G) Expression of
Nv-mef2. (C) Expression begins in individual cells distributed
broadly around the circumference of the blastula. (D,E) Throughout the
development of the planula, expression remains confined to epidermal cells.
(F,G) As development continues, Nv-mef2 expression comes to be
preferentially expressed at the oral pole (arrows), particularly along the
surface of the developing tentacle buds (tb) and tentacles (tn).
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