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Fig. 7. Nv-snailA and NvsnailB. Phylogenetic analysis and gene
expression. (A) Alignment of 105 amino acids containing repeated
C2H2 zinc-finger motifs. (B) Phylogeny of snail
sequences. The tree was constructed and labeled as in
Fig. 3. Sequences included in
the analysis are: escargot, Drosophila, residues 344-446;
Kruppel, Drosophila, residues 250-354; Kruppel-like protein
(zinc finger protein 443), Homo, residues 449-477 plus 505-580;
scratch, Drosophila, 498-600; scratch1, Homo, 222-324;
snail, Drosophila, residues 280-383; snail, Podocoryne,
residues 226-238; snail 1, Homo, residues 154-257; snail 2,
Homo, residues 159-261; snail A, Nematostella, residues 156-258;
snail B, Nematostella, residues 154-256. The tree is drawn as though
rooted using the Kruppel clade as an outgroup. (C-J) Expression of
Nv-snailA through development. (C) Nv-snailA transcript is
not detectable at early cleavage stages. (D) Expression becomes visible in the
late blastula at the site of the blastopore. (E) The embryo initiates
gastrulation at the site of Nv-snailA expression. (F) The first cells
that invaginate into the blastocoel express Nv-snailA. (G) Expression
continues throughout the process of gastrulation as cells move in to the
blastocoel. (H-J) All of the cells of the gastrodermis, but none of the cells
of the epidermis express Nv-snailA up through the polyp stages. The
asterisk indicates the site of the mouth. The expression of Nv-snailB
is indistinguishable from Nv-snailA. Arrows in G,H,J indicate
endoderm.
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