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Fig. 1. PCC spindle formation in a SCNT-reconstructed LoTM zygote, 2 hours after injection of the donor LoTM cumulus cell. Red, DNA; green, ${alpha}$ -tubulin in confocal images. (A) Chromosome condensation and microtubule assembly into a normal PCC spindle. (i) Chromosomes in the middle of the PCC spindle. (ii) Another reconstructed zygote with chromosomes in the middle of the PCC spindle. (iii) Polarized microscopy view of a normal PCC spindle. (B) Chromosome condensation but improper capture onto normally assembled microtubules. (C) Chromosome condensation and microtubule assembly into an abnormal PCC spindle. (D) Chromosome condensation without microtubule assembly. (E) Non-condensed chromosomes and microtubule assembly into an abnormal PCC spindle. (F) No chromosome change and no microtubule assembly (arrow shows first polar body). (G) Normal separation in anaphase of a SCNT-reconstructed LoTM zygote, 2 hours after activation. (H) Abnormal separation of chromosomes in multiple directions on a diffused microtubule assembly, 2 hours after activation.

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