|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Effects of hyperglycemia on iNOS/eNOS distribution and NO production, and
rescue by NO donor. The ability of NOC18 to restore normal vascular morphology
of hyperglycemic treated conceptuses was evaluated by PECAM1 staining at 9.5
dpc: (A) hyperglycemia and (B) hyperglycemia plus NOC18. Morphometric analysis
of images from PECAM1-stained yolk sacs showing vessel diameter distribution
(C). Graphs of ERK2 normalized data for iNOS (D) and eNOS (E) protein
expression in pooled 7.5, 8.5 and 9.5 dpc conceptuses. The broken lines
represent the hyperglycemic condition, whereas the unbroken black lines
represent the control (n=4, *P<0.05,
**P<0.01). The data (mean±s.e.m.) is relative to
the control 7.5 dpc levels. Above each graph is a representative western blot
of iNOS and eNOS at 8.5 dpc. (F) Phase-contrast image of the endoderm of a 7.5
dpc conceptus showing the intact endodermal surface. (G) DAF-FM fluorescence
of the corresponding area of a conceptus cultured in normoglycemic conditions.
(H) DAFFM fluorescence of a similar area of a conceptus cultured in
hyperglycemic conditions. (I) Representative western blot of the effect of a
NO donor on NOS protein distribution of glucose treated conceptuses at 8.5 dpc
and graph of ERK2 normalized averaged data for eNOS and iNOS protein
expression (white columns represent eNOS and black columns represent iNOS;
averages of two experiments). Nml, normoglycemic; HG, hyperglycaemic; HG +
NOD, hyperglycemic + NOC-18.
|
