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Fig. 4. Delay of the secondary ossification center in the Vhlh null growth
plate. (A-D) Hematoxylin and Eosin staining of histological sections of
control (A,C) and null (B,D) proximal tibia growth plates from 5-day-(A,B),
and 1-month-old (C,D) mice. (E-J) In situ hybridization analysis of
histological sections of tibia proximal growth plates from control (E,G,I) and
Vhlh null (F,H,J) 5-day-old mice, with type X collagen (E,F),
PTH/PTHrP receptor (G,H) and Indian Hedgehog (I,J) cRNA; darkfield images are
shown. (K,L) Articular cartilage of control and Vhlh null animals,
one month after birth, at higher magnification.
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