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Fig. 5. Evidence for a decreased cell proliferation rate within the Vhlh null growth plate. (A,B) Detection of BrdU-labeled chondrocytes in histological sections of E16.5 control (A) and Vhlh null (B) proximal tibia growth plates. (C,D) In situ hybridization analysis with p57kip2 cRNA on histological sections of newborn control (C) and Vhlh null (D) growth plates; darkfield images are shown. (E) Percentage of BrdU-labeled cells; bars represent mean percentages (±s.d.) of BrdU-labeled chondrocytes in Vhlh null and wild-type (WT) growth plates at E14.5, E15.5 and E16.5, separated into proliferating zone (PZ) and resting zone (RZ). Statistical differences in each zone were identified using the unpaired t-test. **P<0.01.





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