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Fig. 6. (A,B) Evidence for stabilization of HIF1{alpha} in the Vhlh null growth plate. Immunohistochemical detection of HIF1{alpha} in cryosections of newborn control (A) and Vhlh null (B) proximal tibia growth plates. (C-F) Evidence for an increased HIF1{alpha} activity in Vhlh null growth plates. In situ hybridization analyses of histological sections of proximal tibia growth plates from control (C,E) and Vhlh null (D,F) newborns, with PGK (C,D) and VEGF (E,F) cRNA; darkfield images are shown.





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