QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. (A) Evidence for stabilization of HIF1 in Vhlh null primary chondrocytes. Western blot analysis of whole-cell lysate from primary chondrocytes isolated from Vhlh^+f/+f newborn mice and infected with ß-galactosidase (WT) or Cre recombinase (Vhlh null) adenoviral constructs. Levels of Pro564-hydroxylated HIF1 and -tubulin were analyzed. (B) Evidence for increased HIF1 activity in Vhlh null primary chondrocytes. Elisa measurements of soluble VEGF in the supernatant from primary chondrocytes isolated from Vhlh^+f/+f newborn mice and infected with ß-galactosidase (WT) or Cre recombinase (Vhlh null) adenoviral constructs. (C) VEGF, PGK and type II collagen mRNA expression measured by real-time PCR of total RNA isolated from primary chondrocytes isolated from Vhlh^+f/+f newborn mice and infected with ß-galactosidase (WT) or Cre recombinase (Vhlh null) adenoviral constructs.

Return to article