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Fig. 5. Differentiation of tissues other than TVC in Cs-Mesp knockdown embryos. (A) Suppression of Cs-Mesp with specific morpholino did not affect larval morphology or the expression of (B) endoderm-specific histochemical activity of alkaline phosphatase (arrow), (C) epidermis-specific gene Cs-Epi1, (D) pan-neural marker gene Cs-ETR, (E) mesenchyme-specific gene Cs-Mech1, (F) notochord-specific gene Cs-fibrn, and (G) a muscle-specific actin gene Cs-MA1. (H,I) Expression of Cs-MA1 in (H) control and (I) Cs-Mesp knockdown embryos, which are arrested at the 110-cell stage. As indicated by arrows, a pair of B7.5 blastomeres expresses Cs-MA1 both in control and Cs-Mesp knockdown embryos.





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