DEV011140 Supplemental Data

Files in this Data Supplement:

• Figure S1 -

  Fig. S1. Measurement of morphological parameters during development of Medaka embryos. At stage 13, five common spherical embryos with the diameter of 1000±10 µm were chosen, and the vertical and horizontal diameters (Dv and Dh, respectively), the extent of epiboly, and the length of the thickened cell mass along the dorsal midline (L) were measured from stage 13 (13 hpf) to stage 16++ (23 hpf). The shape of embryos are schematized on the top, and standard deviations of the measured values are indicated by error bars in the graph.

• Figure S2 -

  Fig. S2. Construction of model Medaka embryo by computer graphics and comparison with real embryos. Based on the photo-images and the morphological parameters provided in Fig. S1, a model embryo that develops in animation was constructed. The shapes of embryos were roughly mimicked initially by combination of simple forms and subsequently deformed locally for a better fit to the real shape. As the analysis of the paper focuses on brain development, an effort to pursue realism was made more on the anterior part and less on the trunk and posterior parts. (A-L) Images of the real embryos at the stages indicated, and (A'-L') the counterpart model embryos. From stage 13 (A,A') to stage 16 (F,F'), dorsal views are shown, while from stage 17 (G,G') to stage 24 (L,L') lateral views are presented. Scale bars: 100 µm.

• Movie 1 -

  Movie 1. The original data of cell positions on a model embryo at various developmental stages were assembled into a continuous movie. The dots indicate marked cells color-coded according to their contribution to neural subdividions at stage 24, as in Fig. 3. The data were sampled to produce Fig. 3, and used for analysis shown in Figs 4 and 5. The movie visualizes how the cells converge and are organized into precursors of neural subdivisions.