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Fig. 4. Cyclin A2/cdk2 overexpression is sustained throughout early development.
(A) Embryos were injected with RNA encoding cdk2, cyclin A2, cyclin A2 with
cdk2, or Bgal as a control. Embryos were allowed to develop to stage 23, then
immunoprecipitated cdk2 from injected embryos was tested for its ability to
phosphorylate histone H1. (B) Embryos were injected with RNA encoding cyclin
A2, cdk2, cyclin A2 with cdk2 or GFP as control and allowed to develop to
stage 9 (lanes 1-3, 7) or stage 23 (lanes 4-6, 8). Extracts from embryos,
injected as labelled, were western blotted to detect cyclin A2 protein levels.
(C,D) Cyclin A2/cdk2-injected embryos were grown until tailbud stages
(injected side to the right). Tagged cyclin A2 is stable in all tissues on the
injected side (D), including neural tube (nt), notochord (no), muscle (m) and
epidermis (ep); see Hoechst staining of the same section (C).
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