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Fig. 5. Cyclin A2/cdk2 overexpression promotes cell proliferation in the embryonic
epidermis. Embryos were injected into one cell at the 2-cell stage with cyclin
A2 and cdk2 RNA along with Bgal (light blue). (A) Whole-mount immunostaining
with antiphosphohistone H3 antibodies shows an increase in cell proliferation
on the injected side (left). (B-G) Cyclin A2/cdk2-injected embryos were grown
until tailbud stages and then allowed to incorporate BrdU for 1 hour before
fixing (B,C,E; BrdU uptake) red; nuclei stained in blue with Hoechst dye;
bottom left of each panel: ß-gal auto-fluorescence. Epidermis
overexpressing cyclin A2/cdk2 (C) is disorganised and shows increased BrdU
incorporation compared with epidermis on the uninjected side (B). By contrast,
cell proliferation does not differ in the neural tube and muscle between the
injected side (left) and the uninjected side (right) (E). (D,F,G) Number of
BrdU-positive cells expressed as a percentage of total number of nuclei
(Hoechst-positive) were counted in regions of equal side on the injected and
uninjected sides of the embryos. BrdU incorporation is increased in the
epidermis on the injected side compared with the uninjected side of embryos
(D). No significant difference in BrdU incorporation is seen for muscle (F)
and neural tube (G).
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