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Fig. 4. DNA Synthesis during spore germination and recovery to growth phase. Spores
were heat-shocked, and allowed to recover in liquid growth media. (A) The
increase in nuclei and the peak level of the DNA content profile of
mononucleated cells is plotted over the time of the first cell doubling. (B)
DNA synthesis was measured by BrdU incorporation as in
Fig. 3, using growing cells
that had been labeled for one cell cycle as a control. (C) The percentage of
chromosomal DNA synthesis in germinating spores relative to one cell cycle of
growing cells was estimated from the BrdU incorporation in B. The values are
normalized to the amount of chromosomal DNA on the blot, determined by
Southern hybridization as described in Fig.
3. At the 30-hour time point, there was 52% the BrdU incorporation
observed in growing cells after only a 10% increase in the number of nuclei.
At 21-hours, some BrdU incorporation into chromosomal DNA was detectable upon
longer exposures of the blot (arrow).
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