DEV01149 Supplemental Figures

Files in this Data Supplement:

• Figure S1 -

  Fig. S1. Downregulation of apical adherens junctions in the mesoderm during early gastrulation. Wild-type (A,D,G), htl mutant (B,E,H) and pbl mutant (C,F,I) embryos were fixed and processed for transmission electron microscopy. (A-C) Semi-thin sections; the boxes indicate the position of the electron micrographs shown in each column. (D) Apical contacts of mesoderm cells in the wild type; only a few adherens junctions (arrow) are left at the apical lateral borders of the cells. (G) High-power micrograph; the arrowhead indicates a coated pit, a typical feature during the loss of adherens junctions in the mesoderm (Oda et al., 1998). (E,H) apical contact area of mesoderm cells in htl^AB42 homozygous embryo; the arrows indicate a residual adherens junctions. (F,I) Apical regions of cell contacts between the mesoderm cells of pbl³ homozygous embryo are shown. Adherens junctions are absent from the apicolateral cell contacts. Scale bars: 1 mm.

• Figure S2 -

  Fig. S2. Distribution of adherens junction markers during early mesoderm morphogenesis. Embryos were fixed and stained with antibodies against Da-catenin (A-D) or against DE-cadherin (E-G). In wild type (A,C,E) as well as pbl³ homozgously mutant embryos (C), Da-Catenin and DE-cadherin are first concentrated at the apical borders of the mesodermal tube (arrowheads). After the beginning of phase 2, when the mesoderm cells start to migrate dorsolaterally, both markers are redistributed along the entire plasma membranes as well as the cytoplasm (B,F). In embryos homozygous for pbl³ (D,G), redistribution of Da-Catenin and DE-cadherin occurs as in wild type.