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Fig. 2. HTL is required for the attachment of the mesoderm to the ectoderm. Embryos
(stage 7; phase 1 of migration) were fixed and stained with anti-NRT (red) and
anti-TWI (green) antibodies (A,C) or with anti-CD2 antibodies (B,D). (A) In
the wild type, the mesodermal tube is attached to those ectodermal cells that
lie adjacent to the basal cells of the tube (arrowheads). (B) The invaginated
mesoderm cells are aligned along the midline of the embryo, indicated with
arrowheads. (C) In embryos homozygously mutant for
htlAB42, the basal cells of the mesodermal tube fail to
adhere to the ectoderm. (D) Misalignment of mesoderm cells in htl
mutants with respect to the ventral midline (arrowheads in B,D). (B,D)
Projections of a stack of confocal images covering a total of 25 µm from
the ventral surface of the embryo to the interior.
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