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Fig. 2. pbl mutant embryos have reduced numbers of EVE-positive mesodermal
cells. (A,B) Anti-Fasciclin 3 stains and (C,D) anti-EVE stains of wild-type
(A,C) and pbl2/pbl3 mutant embryos (B,D). (A)
At stage 12 in a wild-type embryo, the visceral mesoderm is seen as a band of
cells running along the anteroposterior axis (arrow). (B) In a similarly
staged pbl2/pbl3 mutant embryo, the visceral
mesoderm is evident, although it is less organised (arrow). (C) At stage 11 in
a wild-type embryo, EVE is expressed in segmentally repeated clusters of
dorsal mesodermal cells. The EVE-expressing neuroblasts are out of focus in
this image. (D) In a similarly staged pbl2/pbl3
mutant embryo, the majority of EVE-positive mesodermal cells fail to form. One
EVE-positive hemisegment can be seen in this embryo (arrow). Based on their
position and morphology, the remainder of the EVE-expressing cells are
neuroblasts (arrowheads).
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