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Fig. 5. The morphology of mesodermal cells is defective in pbl mutant
embryos. Control (A-C,E) and pbl2/pbl3 mutant
(D,F) stage 8 embryos in which GFP-Actin was expressed in mesodermal cells
using the twist-GAL4 driver and visualised with an anti-GFP antibody.
(A) A UAS-GFP-Actin/twist-GAL4 control embryo typical of the stage
used in this morphological analysis, between the first two waves of mitosis in
the mesoderm. (B) Cross-section of a control embryo expressing GFP-Actin in
mesodermal cells. Embryos were oriented so that the leading mesodermal cells
were parallel to the plane of the microscope. The white line indicates the
plane of focus seen in C,D. The black line indicates a deeper plane of focus
seen in E,F. (C,D) Projections of 1 µm optical sections of mid-stage 8
control (C) and pbl2/pbl3 (D) embryos showing
the morphology of migrating mesodermal cells at the leading front. (C) Cells
in a control embryo exhibit numerous protrusions (arrows) in the direction of
migration and appear dissociated from each other. (D) Cells in a
pbl2/pbl3 embryo extend far fewer protrusions
(arrow) and appear more tightly adhered to their mesodermal neighbours. (E)
Mesodermal cells in a control embryo appear more rounded with numerous
intercellular gaps (arrowhead) present. (F) Cells in a
pbl2/pbl3 embryo appear more tightly packed and
are less rounded, with fewer intercellular gaps. Scale bars: 10 µm.
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