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Fig. 1. Three-day regenerating tails in longitudinal (A-C,H) and transverse (D-G) sections immunostained with 12/101 mAb and counterstained with Haematoxylin. (A) Low-power magnification showing neural ampulla (NAmp), regenerating notochord (R.Not) and blastema (i.e. visibly undifferentiated cells) formation close to the cut surface. (B) Enlarged view of boxed area in A, showing in detail the NAmp. (C) Enlarged view of boxed area in A showing R.Not. (D) Stump transversal section, proximal to amputation surface, showing notochord (Not), spinal cord (SC) and muscle (Mus). (E) Detail of the enlargement of the ependymal layer in the NAmp. (F) Transverse section distal to amputation level showing R.Not and the surrounding undifferentiated blastema cells. (G,H) Degenerating muscle (D.Mus) (black arrows) near the amputation level. Scale bars: 100 µm.





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