|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. D-h expression is activated by a Hh-response element. (A) A third
instar leg imaginal disc depicting expression of the DV-h stripes
(blue) relative to ac expression (green) and the compartment
boundary. The D-h and V-h stripes occupy distinct registers
relative to the AP boundary. (B) (Top) map of the h locus showing the
position of the D-h enhancer. Below is shown a more detailed map of
the D-h enhancer. Fragments tested in reporter constructs are shown
beneath the detailed map: full-length D-h enhancer (light blue) and
the Hh response element (HHRE, dark blue), which has two Ci binding sites and
the repression element (REPE, red; see text and
Fig. 3). The CMB site in the
REPE is described in the text and in Fig.
8. (C) Sequence of two Ci-binding sites present in the HHRE.
Underlined bases were altered in the Ci1, Ci2 or Ci1&2 double mutant (see
Materials and methods). (D,E) lacZ expression in leg imaginal discs
carrying: D-h-lacZ (D) and HHRE-lacZ (E). (F-G)
lacZ expression directed by HHRE-lacZ site mutants: Ci1
mutant (F), Ci2 (G) and Ci1&2 (H).
|
