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Fig. 2. Exogenous Lmx1a induces ectopic roof plate in the chick developing
spinal cord. Expression of MafB (A-E), Msx1/2 (G-K) (both red), Gdf7
(L,M), Bmp4 (N,O), and Wnt1 (P,Q) (all blue) in
unelectroporated embryos (A,G,L,N,P) and embryos electroporated with either
Lmx1a-IRES-EGFP (B,H,M,O,Q) or EGFP alone (D,E,J,K). All pictures are from
stage-24 embryos, 48 hours after electroporation Arrowheads point to ventral
boundary of ectopic domains of expression of roof plate markers induced by
exogenous Lmx1a. (F) Quantitative analysis of dorsal MafB-positive cells in
embryos electroporated with Lmx1a and EGFP alone. (R-U) Expression of
MafB (red) and Pax7 (blue) (R,T) or MafB and Pax7 together with GFP (green)
(S,U) in intermediate neural plate explants (I.E.), electroporated with
Lmx1a-IRES-EGFP (T,U) or EGFP alone (R,S). (V) Quantitative analysis of
MafB/Pax7 double-positive cells in intermediate explants electroporated with
Lmx1a and EGFP alone.
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