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Fig. 4. Lmx1a withdraws neural progenitors from the cell cycle. GFP-fluorescence (green) in chick neural tubes 48 hours after electroporation (h.a.e.) with Lmx1a-IRES-EGFP (A) or EGFP alone (B). Arrows point to electroporated cells located in the pial layer of the neural tube (A). (C) Schematic representation of the neural tube divided into three layers to quantify distribution of electroporated cells along the medio-lateral axis. (D) Quantification of percent of electroporated cells found in each of these layers. (E-H). BrdU incorporation (red) in chick developing spinal cords 18 h.a.e. (E,F) and 36 h.a.e. (G,H) with Lmx1a-IRES-EGFP. Arrows point to BrdU-positive cells expressing exogenous Lmx1a. Insets show higher magnifications of boxed regions. (I-L). BrdU immunohistochemistry (red) alone (I,K) or together with Lmx1a visualization (green) (J,L) in E10 wild-type (I,J) and dreher (K,L) mouse embryos. BrdU/Lmx1a-double-positive cells (yellow) are indicated by arrowheads (L). Insets show higher magnifications of the dorsal midline regions. Bars equal 40 µm. (M,N) Quantification of proliferation (M) of Lmx1a-positive cells and measurement of Lmx1a-positive area (in arbitrary units) (N) in transverse sections of neural tubes taken from E10.0 wild type and dr^J/dr^J embryos.

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