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Fig. 8. Effect of Bmp4 overexpression on Lmx1a expression and roof plate development. (A-D) Chick neural tubes expressing exogenous Bmp4 24 hours after electroporation. Visualization of expression of Lmx1a (A) and MafB (C) (both red) alone or together with GFP (green) (B,D). Arrowheads show the ventral boundary of expanded expression domains of both Lmx1a and MafB in neural tubes electroporated with Bmp4. (E-L) Wild-type (wt exp) (E-H) and dreher (dreher exp) (I-L) explants cultured without Bmp4 (E,F,I,J) or with Bmp4 (G,H,K,L). Visualization of expression of Lmx1a (E,G,I,K) and MafB (F,H,J,L).





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