DEV01153 Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Time-lapse sequence of a Lilium longiflorum (lily) pollen tube growing facing an extracellular NO point-source (SNAP on agarose; left on the image). Pollen tube slows as it moves into the NO-gradient, but direction proceeds unchanged for ~12 minutes. A new growth axis then starts to be defined, forming a sharp right angle from the original axis (97.7±3.6°, n=28). The pollen tube then regains normal growth rate (16-20 minutes). Scale bar: 30 mm

• Movie 2 -

  Movie 2. Time-lapse sequence of a pollen tube being challenged with a diluted NO artificial source in the presence of sildenafil citrate (Viagra^TM) (numbers in the top right-hand corner represent minutes after detection of the response). Using these diluted sources, most pollen tubes do not show any response, often growing into the pipette. For this experiment, pollen tubes were first incubated on standard medium and challenged with the diluted NO source. If a pollen tube showed no response, i.e. if it was demonstrated to be insensitive to such low amounts of NO, the medium was perfused with sildenafil citrate and the same pollen tube is challenged with the same NO source. Despite the lower amount of NO, reverse re-orientation angles were observed in the presence of sildenafil citrate (109.8±9.8°, n=9) showing a sensitization effect, from unresponsive to the peak response.