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Fig. 3. The DAF-2DA-positive organelles are peroxisomes. (A-D) Confocal images of
growing lily pollen tubes incubated in DAF-2DA (green) and in
organelle-specific dyes (red). No co-localization was found in the
endomembrane system (A, BodipyTR), mitochondria (B, Rhodamine 123) or acidic
organelles (C, LysotrackerRed). (D) Peroxisomes were then tagged by transient
transformation of pollen grains with a construct containing the
pollen-specific LAT52 promoter driving an ECFP-peroxisome targeting signal
(PxTS) fusion. Pollen tubes were observed 10 hours after germination. The
NO-producing organelles (DAF-2, green) show an almost complete co-localization
with the ECP signal (PxTS-CFP, blue), as shown in the merged image. Scale
bars: 15 µm.
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