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Fig. 6. ecd is expressed in specific steroidogenic, non-steroidogenic and
reproductive organs. (A-F) The anti-Ecd antibody strongly stains the lateral
lobes of a third instar ring gland (A); a confocal image (B) shows that Ecd is
in the cytoplasm. Low signal is seen in the ring gland during the second
instar (C, arrows). Activity of an ecd-lacZ reporter in the ring
gland of second instar (D) and late third instar (E) larvae is visualized with
X-gal staining. Arrowhead in D marks the medial corpora allata region. (F)
Anti-Ecd antibody staining shows Ecd expression targeted to the lateral ring
gland of a Feb36-Gal4; ecd2, P[UAS-ecd] second instar
larva. (G-I) A third instar CNS shows moderate Ecd levels (G) compared with
the lateral ring gland (A). Ecd is abundant in the eye-antennal (H) and wing
(I) imaginal discs. (J-N) The anti-Ecd antibody shows strong expression in
both the ovary (J) and the testis (K) of third instar larvae. High levels of
Ecd occur in the nurse cells of stage 10 egg chambers (L, arrow). The same
stage nurse cells also accumulate an ecd transcript, as shown by in
situ hybridization (M). Ecd is present primarily on the apical end of the
adult testis (N). Except for F, staining was performed on
white1118 animals.
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