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Fig. 1. Experimental procedures. (A) Mesoderm migration on conditioned substratum.
(Left) Mesoderm cells migrate on the extracellular matrix (ECM) of the BCR
towards the animal pole (AP) (arrow). Blue, prospective ectoderm; pink,
mesoderm (M); yellow, endoderm (E). (Right, top) A strip of BCR (bracket in
left figure) is explanted at stage 10, and cultured for 1 hour with its
matrix-forming surface down. (Right, bottom) The ECM of the BCR has become
deposited at the bottom of the dish. A mesodermal explant (M) placed on the
conditioned substratum migrates (arrow) to the animal pole position. (B)
Construction of trPDGFA. The sequence corresponding to amino acid residues
198-227, that contain the matrix binding motif, was deleted from the
lfPDGFA.
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