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Fig. 1. Keratinocyte-specific Cre expression leads to pRb ablation in vivo. (A) Schematic representation of the wild-type, the floxed (F19) and the inactivated Rb allele ({Delta}19). Exons are indicated as numbered boxes and loxP sites as triangles. Rb18 and Rb19 indicate the primers used to characterize the different Rb alleles. (B-E) ß-Galactosidase staining (blue) identifies Cre activity in X-Gal stained tissue sections from epithelia of double transgenic R26R:K14cre animals in epidermis (B), tongue (C), thymic epithelium (D) and hard palate (E). (F) Cre-mediated recombination detected by PCR analysis on tails RbF19/F19;K14cre mice. (G) Immunoblot analysis for pRb in RbF19/F19 keratinocytes 48 hours post-infection with Ad-GFP or Cre-coding adenovirus. (H) Immunoblot analysis for pRb family members on primary keratinocytes derived from newborn mice with the indicated genotypes. Scale bars: 150 µm.





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