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Fig. 8. VEGF induces SCL-expressing cells mainly through the FLK1 receptor. (A)
Wild-type, Flk1–/– and
Flt1–/– ES clones were differentiated in serum
or serum-free conditions, in the presence of BMP4 and VEGF as shown. Day 5-6
EBs were analyzed for CD31 and TER119 expression. The percentage of TER119 and
CD31-positive cells are shown. One representative experiment out of three is
shown. (B) Flk1–/– and
Flt1–/– EB cells generated in the presence of
FCS (black bar), SR+BMP4 (white bar), or SR+BMP4+VEGF (grey bar) were
re-plated in a semi-solid medium as described in the Materials and methods.
Hematopoietic colonies were counted 5-7 days after re-plating. No
hematopoietic colonies formed when EB cells from SR were re-plated, thus not
shown. Error bars indicate standard deviations from triplicate plating. One
representative experiment out of three is shown.
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