spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 9. TGFß1 and activin A in VEGF-mediated expansion of SCL-expressing cells, hematopoietic and endothelial cell differentiation. Day 6 Scl+/CD4 EB cells generated in serum-free conditions in the presence of BMP4; BMP4+VEGF; BMP4+VEGF+TGFß1; or BMP4+VEGF+activin A were subjected to three-color analyses for FLK1, CD4 and hematopoietic or endothelial markers. (A) The percentage of FLK1+CD4– (lower right), FLK1+CD4+ (upper right) or FLK1–CD4+ (upper left) cells is shown. Statistical values are shown on the right. (B) The percentage of TER119 and CD31-positive cells is shown. (C) EB cells generated in the presence of FCS; SR+BMP4; SR+BMP4+VEGF; SR+BMP4+VEGF+TGFß1; or SR+BMP4+VEGF+activin A were subjected to hematopoietic re-plating. No hematopoietic colonies formed when EB cells from SR were re-plated, thus not shown. Hematopoietic colonies were counted 5-7 days after re-plating. Error bars indicate standard deviations from triplicate plating.





Right arrow Return to article