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Fig. 5. Phenotypes of zebrafish embryos injected with mkp3 and
mkp3::C292S RNA. (A-C) Somitogenesis stages of zebrafish embryos
uninjected (A), injected with mkp3 (B) or with
mkp3::C292S (C). Injected embryos display anterior defects (red
arrowhead). (D-I) At 24 hpf, mkp3-injected embryos (E) exhibit
anterior truncation and expanded trunk-tail region, while
mkp3::C292S-injected embryos, show trunk-tail defects (F) (compare
with uninjected in D). (G-I) Staining for gata1 at 24 hpf reveals
ventralization of mkp3-injected (H) and dorsalization in
mkp3::C292S-injected embryos (I); arrows indicate region of
gata1 staining (compare with G, uninjected). (J-Q) Early dorsoventral
markers are disrupted in injected embryos. bmp4 is activated in
mkp3-injected embryos (J,K, 70% epiboly), while chordin
expression is suppressed (L,M, shield stage). Conversely, ectopic expression
of mkp3::C292S expands expression of chordin at shield stage
(N,O; arrowheads indicate the boundary of chd expression), and
expression of neural markers en3 and krox20 (P,Q) at
one-somite stage. MHB, mid/hindbrain boundary; r3 and r5, rhombomeres 3 and
5.
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