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Fig. 7. Expression of FGF genes in ich mutant embryos and rescue of
phenotype by FGF. (A-M) Mutant embryos derived from ich-/-
females and wild-type embryos derived from ich+/- females
are shown as animal pole views. Initial expression of fgf3 and
fgf8 is strongly reduced at sphere stage in mutant embryos (B,D)
compared with wild-type embryos (A,C), where the double arrows mark dorsal
expression. (E-M) Expression of fgf3 continues to be reduced in
mutant embryos at 30% (E,F) and 50% epiboly (I,J). By contrast, fgf8
expression is detected at 30% epiboly around the margin (G,H), and continues
in mutant and wild-type embryos at 50% (K,M). (N-O) Overexpression of
fgf3 (compare N with O) or fgf8 (compare N with P) partially
rescues ich embryos (asterisks indicates rescued embryos). (Q) Rescue
of ich by injection of FGF genes and ß-catenin. Both
fgf3 and fgf8 injections resulted in partial rescue of
mutant embryos. RNA encoding the nuclear localized form of
ß-galactosidase (nß-gal) or zebrafish ß-catenin was injected
into ich embryos as a negative and positive control, respectively.
Phenotypic classification was slightly modified from Kelly et al.
(Kelly et al., 2000): class 1
embryos are the most ventralized, lacking trunk and head; class 1a develop
partial trunk and spinal cord but no hindbrain or more anterior structures;
class 2 develop spinal cord and hindbrain but not midbrain, forebrain or eyes;
class 3 develop incomplete anterior brain and eye structures; class 4 develop
a complete AP axis but no notochord; class 5 appear normal at 24 hpf.
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