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Fig. 4. HOM-C proteins accumulate in the proper register in embryos with ectopic DFD and DISCO but lacking TSH. Stage 12 embryos are shown. In A-C, we show Antennapedia (ANTP) accumulation; the arrowhead marks the beginning of ANTP accumulation in posterior t1. (A) Wild-type embryo. (B) Homozygous tsh⁸ embryo. (C) tsh⁸, UAS-disco, UAS-Dfd/tsh⁸; arm-Gal4/+ embryo. In D-F, Ultrabithorax (UBX) accumulation is shown with the arrowhead marking posterior t3. (D) Wild-type embryo. (E) Homozygous tsh⁸ embryo. (F) tsh⁸, UAS-disco, UAS-Dfd/tsh⁸; arm-Gal4/+ embryo. Note that the register of expression is the same in all cases. The tint to the embryos in B,C,E, F is a consequence of in situ localization of tsh mRNA to unequivocally identify tsh mutant embryos.

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