DEV01165 Supplemental Figures

Files in this Data Supplement:

• Figure S1 -

  Fig. S1. 293T cells (0.6´10⁵ cells) were transfected with the indicated amounts of pMX-Wnt7a-IRES-GFP (Wnt7a), pMX-Wnt7aHA-IRES-GFP (Wnt7aHA) or corresponding empty vector (–), with (+) or without (–) 0.05 mg of pCS2+-Fz5, together with 0.025 mg of TOP-FLASH or FOP-FLASH, with the FuGENE 6 transfection reagent. Data are the mean+s.d. of values from three samples.

• Figure S2 -

  Fig. S2. Expression of HA-tagged Wnt7a or Axin partially suppressed neuronal differentiation in the cortical NPCs. NPCs isolated from E11.5 were infected with a retrovirus encoding either GFP alone, or GFP together with Wnt7a, Wnt7aHA or Axin. The cells were then cultured in suspension for 3 days in the presence of FGF2. The resulting neurospheres were dissociated and plated onto coverslips coated with poly-D-lysine. The cells were incubated for 2 days with  FGF2, and then the percentage of TuJ1⁺ cells among GFP⁺ cells was determined. *P< 0.01, **P< 0.0025, ***P< 0.0005, versus control.

• Figure S3 -

  Fig. S3. NPCs were infected with a retrovirus encoding GFP together with the S33Y b-catenin. The percentages of Nestin⁺, TuJ1⁺ and GFAP⁺ cells among GFP⁺ cells were determined after culture for 5 days in the absence of FGF2. More than 90% of active b-catenin-expressing cells became TuJ1⁺, whereas 5% and 3% of these cells were Nestin⁺ and GFAP⁺, respectively. Data are the mean+s.e.m. of values from four samples. Similar results were obtained in three independent experiments.