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Fig. 1. SHR::GFP does not move from phloem companion cells (CC), where the native SHR promoter is not active. Longitudinal (A-C) and transverse (D,E,G) confocal images of GFP fluorescence of pSUC2::GFP (A), pSUC2::GFP_ER (GFP_ER contains ER targeting and retention signals) (B,D), pSUC2::SHR::GFP (C,E) and pSHR::SHR::GFP (G) transgenic roots. (F) In situ hybridization with GFP antisense probe on transverse section from pSHR::SHR::GFP transgenic root. The SUC2 promoter is active in the phloem CC (B and arrows in D). Non-targeted GFP can move from the CC to the epidermis (A), while the fusion protein SHR::GFP does not leave the CC (C and arrows in E). The native SHR promoter is not active in the phloem CC (arrows in F), yet the SHR::GFP fusion protein can be found throughout the stele and in the endodermis when transcribed under the same promoter (G). Arrowheads in D and E indicate protoxylem. ep, epidermis; co, cortex; en, endodermis; st, stele. Scale bars: 50 µm in A-C; 25 µm in D-G.

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