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Fig. 3. SHR::GFP does not move from the epidermis even when supernumerary layers are present. Longitudinal confocal images (A-D) and transverse section (E) of pGL2::YFPER (inset in A), pWER::YFPER (inset in C), pGL2::SHR::GFP (A,B) and pWER::SHR::GFP (C-E) transgenic roots. pWER::SHR::GFP induces a pattern perturbation resulting in supernumerary layers (C,E), while pGL2::SHR::GFP does not alter the pattern (A). SHR::GFP does not appear to move from the epidermis in either pGL2::SHR::GFP (B) or in pWER::SHR::GFP (D) transgenic roots. Arrow in the inset of C indicates ep/LRC initial. ep, epidermis; co, cortex; en, endodermis; sn, supernumerary layers. Scale bars: 50 µm in A,C,E; 25 µm in B,D.





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