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Fig. 4. SCR is required for pWER::SHR::GFP-mediated ectopic divisions and
movement of SHR::GFP from the epidermis occurs in a scr mutant
background. Longitudinal confocal images of pWER::SHR::GFP transgenic
roots in a scr-4 background (A, red channel only) and (B) a
pSHR::SHR::GFP transgenic root in scr-1 background (inset in
B), and in situ hybridizations with GFP antisense probe on transverse
sections from pWER::SHR::GFP transgenic roots in wild-type (C) and
scr-4 (D) backgrounds. The resulting radial pattern (A) is identical
to that of scr (Di Laurenzio et
al., 1996). The fusion protein SHR::GFP is found in the epidermis
as well as in the mutant ground tissue layer (B), whereas no RNA expression of
SHR::GFP is detectable in the mutant layer of the scr-4 root
(D), indicating movement of the fusion protein from the epidermis to the
mutant layer. SHR::GFP is detected both in the nuclei and in the cytoplasm of
the mutant layer, when moving either from the epidermis [as in
pWER::SHR::GFP in scr-4 (B)] or from the stele [as in
pSHR::SHR::GFP in scr-1 (inset in B)]. mut, mutant layer of
scr; LRC, lateral root cap; ep, epidermis; sn, supernumerary layers;
st, stele. Arrowheads in the inset indicate the mutant layer. Scale bars: 50
µm in A; 25 µm in B-D.
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