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Fig. 3. spry2 is a novel member of the fgf8 synexpression group.
Comparison of the expression pattern of spry2 (A,B,D,F,H,J,L,N,P,R,T)
with fgf8 (C,E,G,I,K,M,Q,S), fgf3 (O), spry4 (U),
sef (V), erm1 (W) and pea3 (X). (A,B)
spry2 transcripts in 32-cell stage embryos. (C,D) Late blastula stage
expression of fgf8 (C) and spry2 (D). (E,F) Midgastrula
stage expression of fgf8 (E) and spry2 (F); black arrowhead
indicates margin; black arrow indicates presumptive midbrain-hindbrain region;
white arrow indicates axial hypoblast; white arrowhead indicates presumptive
forebrain. (G-J) Five-somite stage expression of fgf8 (G,I) and
spry2 (H,J). (K,L) Expression of fgf8 (K) and spry2
(L) at the 16-somite stage. (M-P) Expression of fgf8 (M),
fgf3 (O) and spry2 (N,P) at 36 hours. Arrowheads indicate
rhombomere boundaries; arrows indicate anterior otic vesicle. (Q-T) Expression
of fgf8 (Q,S) and spry2 (R,T) at 48 hpf in the
neurohypophysis (Q), adenohypophysis (R), the apical ectodermal ridge (S) and
mesenchyme of the pectoral fin (T). (U,V) Five-somite stage expression of
spry4 (U), sef (V), erm1 (W) and pea3 (X).
(A,C,D,G,H,K-R, U-X) Lateral views; (B) animal pole view; (I,J,S,T) dorsal
views. Anterior is upwards in C-F and towards the left in G-X. ba, branchial
arches; dd, dorsal diencephalon; hpm, heart primordia; mhb, midbrain hindbrain
boundary; os, optic stalk; r2/r4, rhombomeres 2 and 4; som, somites; tb, tail
bud; tel, telencephalon.
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