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Fig. 2. Defective ovulation in par-3(RNAi) worms. Images from time-lapse
Nomarski video microscopic observations of meiotic maturation and ovulation in
the wild type (left) and a first ovulation in par-3(RNAi) (right).
Real time (minutes:seconds) is indicated in the top left corner, with the zero
time point corresponding to the time that nuclear envelope break down (NEBD)
was observed (black arrows). An oocyte (o), the oocyte nuclear envelope (ne),
the sheath (sh) and the spermatheca (sp) are indicated. The oocyte enters the
spermatheca in the wild type at 0:45 and is transferred to the uterus at 1:10.
However, in par-3(RNAi) worms, the oocyte remains in the proximal
gonad even 4:28 after the NEBD. The contraction of sheath cells and distal
extension of the spermatheca was observed in both worms (arrowheads). Scale
bar: 10 µm.
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