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Fig. 3. Shh protein is detectable in epithelium and mesenchyme of the developing
tooth germ in a wider distribution than epithelial-restricted Shh.
(A,C,E,G) Immunohistochemistry; (B,D,F,H) section in situ hybridisation. (A,B)
E11.5 epithelial thickening. (C,D) E12.5 early bud. (E,F) E13.5 late bud.
(G,H) E14.5 cap. Shh protein is undetectable in the diastema in the presence
of epithelium. (I,J) E11.5 mandibles cultured for 24 hours. (I) Section
through the early molar epithelial thickenings (arrows indicate Shh protein in
odontogenic epithelium). (J) Section through the diastema demonstrating a lack
of detectable Shh protein. (K,L) Immunohistochemistry. Timed-culture of
E11.5-isolated mandibular mesenchyme demonstrating the dynamics of Shh
detection in the diastema. (K) Shh is first detectable after approximately 20
hours. (L) Shh is strongly detected in the diastema after 24 hours. Shh
immunohistochemistry does not cross-react with Ihh. (M,N) E16.5 mandibular
condyle. (M) Ihh; (N) adjacent section stained for Shh demonstrating no
cross-reaction with Ihh. mc, Meckel's cartilage; t, tongue. Scale bars: in A,
50 µm for A-F; in G, 150 µm for G,H; in I, 600 µm for I,J; in K, 600
µm for K,L; in M, 500 µm for M,N.
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