vß3 integrin-dependent endothelial cell dynamics in vivo

DEV01160 Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Early vascular dynamics in a normal HH stage 8 quail embryo. Epifluorescence images of endothelial cells and structures are shown in white. Composite frames were taken every 9.2 minutes. The movie has a display rate of 15 frames/second. Fig. 1 contains still images from this movie with inverted colors.

• Movie 2 -

  Movie 2. High magnification movie displaying 0.16 mm² areas analyzed in normal and LM609-perturbed embryos. The movie contains four separate panels that show the vascular dynamics occurring within defined areas in two normal and two LM609-perturbed embryos. Again, endothelial cells and structures are white. Frames were taken every 13.8 minutes for the normal embryos and every 12.9 minutes in the experimental set displayed here. The display rate is 6 frames/second. Figs 2 and 6 contain still images from the upper right and lower left panels, respectively. To provide better contrast, the color in the still images is the inverse of those seen in the movies.

• Movie 3 -

  Movie 3. Cell trajectories of untreated primordial endothelial cells (PEC). The progressions of a number of trajectories from PECs are displayed as continuous lines. Endothelial cells and structures are shown in white. Each frame is taken 13.7 minutes apart. The display rate is 10 frames/second.

• Movie 4 -

  Movie 4. Extensions and retractions between cell clusters. The dynamics of two cell clusters are displayed in a three-paneled movie. A cell from each cluster sent out an extension, one of which later retracted, that ultimately resulted in a new connection within the primary vascular plexus. The left panel is DIC and the middle is epifluorescence with endothelial structures in black, while the right panel is a merged composite of the previous two with endothelial cells in red. All of the frames are displayed in Fig. 4. Frames were acquired 13 minutes apart. The display rate is 6 frames/second.

• Movie 5 -

  Movie 5. Reduced vascular dynamics in a HH stage 8 quail embryo injected with LM609. Epifluorescence images of endothelial cells and structures are shown in white. Composite frames were taken every 12.9 minutes. The movie has a display rate of 10 frames/second. Fig. 5 contains still images from this movie with the colors reversed.