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Fig. 1. Stages of early vasculogenesis. (A-C) Taken from Movie 1 at
http://dev.biologists.org/supplemental.
Changes in the Cy3-QH1 pulse labeled primordial endothelial cell population
over a 12 hour time period, with corresponding DIC images (insets). (D) The
merged image of the pulse-labeled PECs (red) and the post-experimentally
Cy2-QH1 labeled vasculature (green). The red and green open circles mark the
same PECs and endothelial structures whose movements are followed in each
panel. Note the extensive lateral-to-medial movement of those foci lying most
distal to the midline axis (red lines). By comparison, those in a more
proximal position show lesser displacements. The cyan colored box indicates
the field that is enlarged in Fig.
2 and serves as a frame of reference between panels. The box is
400x400 µm with the upper right corner centered on the lateral edge
of the third formed somite (yellow asterisks, inset), 160 µm from the
midline. All images are from the ventral aspect. The yellow arrowheads in C
(inset) indicate the developing pronephros. sv, sinus venosus; nf, neural
folds; ao, area opaca; nc, notochord.
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