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Fig. 2. Cell dynamics establishing the primary vascular plexus. Time points 1-11
show the cell configurations, at hour intervals, within the area delineated by
the cyan colored box in Fig. 1.
Each panel represents a 400x400 µm area enlarged from individual
frames in Movie 1, and are shown in Movie 2 (see
http://dev.biologists.org/supplemental).
As a reference, the upper right-hand corner is centered on the lateral edge of
the third somite pair 160 µm from the midline. The final panel is a merged
image composed of the Cy3-QH1, experimental PEC label (red) and the
post-experimental labeling of the vasculature using Cy2-QH1 (green). The open
circles enclose the same endothelial structures on each panel to ease
following movements. Note the lateral-to-medial movement of all denoted PECs.
Green circles represent new features entering the field of view from a lateral
position. Blue arrowheads indicate new cellular extensions and connections
with existing polygonal structures. The red arrowheads represent retractions
of vascular cords. In the last panel, a red arrowhead indicates where one
connection once existed between two of the circled areas. In addition, some
vasculature is formed (green) that is not recorded (red/yellow).
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