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Fig. 5. Blocking 
vß3 integrin activity disrupts early vasculogenesis.
(A-C) Taken from Movie 5 (see
http://dev.biologists.org/supplemental),
show changes in endothelial structures over a 10 hour period within an embryo
injected with LM609, an vß3 blocking antibody. (D) The merged
image of the Cy3-QH1, pulse labeled (red) and the post-experimentally labeled
vasculature (green). In D, the disruption of the dorsal aortae and the
polygonal network is clearly visible (see
Fig. 1 as a normal reference).
Clusters of vascular endothelial cells, characteristic for earlier stages (A
in Figs 1 and 5) persist,
lacking protrusions and connections. The red and green open circles indicate
the same endothelial structures on each panel. By comparing with
Fig. 1, a substantial reduction
in the lateral-to-medial vascular drift is observed (red bars). The
vasculature marked by green open circles is fused while being drawn into the
developing endocardium. This motion seems to be unaffected by LM609 exposure.
The cyan box delineates the field that is enlarged in
Fig. 6 and serves as a frame of
reference between panels. The position of this area as well as the markers,
with the exception of one (is, injection site), is the same as in
Fig. 1.
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