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Fig. 7. nlk MO affects expression of MHB and diencephalon markers. Embryos
were injected with nlk or wnt8b MOs. At bud stage, embryos
were stained with either a mix of opl, pax2 and tbx6 (A,B,C;
dorsal view, anterior to top), or with en2 (D,E,F; dorsal view,
anterior to left). At 7 somites, embryos were stained with either en2
(G,H,I; dorsal view, anterior to left) or pax6 (J,K,L; dorsal view,
anterior to top). Although expression of opl and pax2 are
unaffected in wnt8b morphants (B) compared with wild type (A),
nlk morphants have a slight reduction in pax2 expression (C;
arrow). en2 expression is reduced in wnt8b MO (E), and is
eliminated in nlk MO (F) compared with wild type (D). At 7 somites,
en2 is expressed in the MHB and midbrain (G). Expression is
substantially reduced in both wnt8b and nlk morphants (H,I;
brackets delineate expression domain). pax6 expression is unchanged
in wnt8b MO (K), but is reduced slightly in nlk MO (L;
brackets indicate AP extent of expression domain).
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