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Fig. 2. Expression of Hemps normalised to Hec-coA compared with Hemps northern analysis. (A) The expression of Hec-coA was compared with the expression of Hec-Ubiq through developmental stages egg, gastrula, mid-tailbud, hatched, competent, 30 minutes post-induction (PI), 4 hours PI, 16 hours PI, 24 hours PI and juvenile. (B) The expression of Hemps through developmental stages egg to juvenile normalised to Hec-coA using quantitative real-time RT-PCR (QPCR). (C) Densitometry analysis of Hemps expression using northern analysis shows a similar expression profile to that derived using QPCR (Eri et al., 1999).





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