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Fig. 3. Validation of the expression levels between normal metamorphosing larvae and anti-Hemps antibody-treated larvae using quantitative real-time RT-PCR (QPCR) analysis at 30 minutes post-induction (PI; A) and 4 hours PI (B). Clones selected are Hec-bcsX1, Hec-meta1, Hec-meta2a, Hec-meta2, Hec-sap, Hec-mbl, Hec-shm, Hec-rab, Hec-nopp34, Hec-hsup and one unknown gene, Ac925 (left to right). Shown are the ratios (treated:normal) derived by the microarrays and QPCR. A ratio >1 represents a gene with expression that is upregulated when treated with anti-Hemps antibody. A ratio <1 represents a gene with expression that is downregulated when treated with anti-Hemps antibody. An asterisk indicates a significant difference between treated and untreated larvae.





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